6/12/2023 0 Comments Vladmir vimr![]() The HutC subfamily represents more then 30% of all GntR members. 2001), no structural information is available for the other three subfamilies of GntR-like regulators. While the structure of FadR alone and in complex with its effector and operator DNA has been recently determined ( van Aalten et al. ![]() According to this analysis, the GntR family has been divided into four major subfamilies, FadR, HutC, MocR, and YtrA, where each subfamily is categorized by a specific type of the C-terminal domain. Recent sequence analysis of the GntR proteins revealed the presence of several distinct groups with different types of the C-terminal signaling domains ( Rigali et al. This output domain is coupled with the C-terminal signaling domain responding to a range of stimuli in the form of different small molecules. The proteins in the GntR family share a characteristic version of the N-terminal winged helix-turn-helix (wHTH) DNA-binding domain. This is true for the GntR family of transcriptional regulators, which gathers close to 2000 members in both bacterial and archael genomes ( Haydon and Guest 1991 Rigali et al. With extensive sequence similarity in the DNA-binding domain, these large families often contain proteins with different types of the effector domains fused to similar DNA-binding domains ( Rosinski and Atchley 1999 Perez-Rueda and Collado-Vides 2000). 1994), GntR ( Haydon and Guest 1991), and AraC ( Gallegos et al. Some of the largest such families ( Perez-Rueda et al. Based on sequence similarity, predominantly in the DNA binding domain one-component systems identified in sequenced genomes are assembled into protein families usually named after the best-characterized member. Signal transduction in prokaryotes is dominated by such systems, where small molecule-binding domains constitute the majority of the input domains and helix-turn-helix (HTH) DNA-binding domains are the most common output domains ( Ulrich et al. The structural comparison of the C-PhnF and UbiC proteins allows us to propose that a similar site in the PhnF structure is adapted for effector binding.Ī protein containing a sensor input domain and a functional output domain is described as a one-component system ( Ulrich et al. C-PhnF shares strong structural similarity with the chorismate lyase fold, which features a buried active site locked behind two helix-turn-helix loops. C-PhnF monomers form a dimer by establishing interdomain eight-strand β-sheets that include core antiparallel and N-terminal two-strand parallel β-sheets from each monomer. The structure represents a mixture of α-helices and β-strands, with a six-stranded antiparallel β-sheet at the core. The C-PhnF structure provides for the first time the scaffold of this domain for the HutC subfamily, which covers about 31% of GntR-like regulators. Members of this family share similar N-terminal DNA-binding domains, but are divided into four subfamilies according to their heterogenic C-terminal domains, which are involved in effector binding and oligomerization. The PhnF protein belongs to the HutC subfamily of the large GntR transcriptional regulator family. Queries to page last revised Thursday 21 November 2019.The crystal structure of Escherichia coli PhnF C-terminal domain (C-PhnF) was solved at 1.7 Å resolution by the single wavelength anomalous dispersion (SAD) method. Move to the home page for the School of Slavonic and East Spectators Vladimir Linka, František HruškaĬonversations with the actors Jiřina Bohdalová (3 minutes 29 seconds), Radoslav Brzobohatý (2 minutes 43 seconds), František Peterka (6 minutes 49 seconds), all in Czech without subtitlesīiographies and filmographies for the director, Ladislav Rychman and the actors Jiřina Bohdalová, Radoslav Brzobohatý, František Peterka and Libuše GeprtováĬentrum Českeho Videa, Bonton Entertainment Group, Brickbox Digital Media Jaroslav Klenot, Jiří Plachý, Oto Rademacher ![]() Photographers Martin Hoffmeister, Jindřich Panáčekīoxers Karel Engel, Jindřich Sejk, Jiří Kasík, ![]() Jana Houkalová, Jana Kopecká, Helena Koudelková-Kaftani,ĭanseurs Jiří Kaftan, Jaroslav Pešek, Jaromir Petřik,ĭriver Jiří Havel, Jaroslav Holeček, Eugen Jegorov, Julie Jenšíková, Jana Nováková, Marie Popelková,ĭanseuses Marcela Březinová, Saša Grossová, Women at fashion show Slávka Hamouzová, Ivana Herglotzova, Women on stairs Ružena Dvorská, Marie Norrová, Marcela Sedláčková Insurance agents Josef Hanuš, Ladislav Hanuš, Ladislav RychmanĬonferenciers Jan Bartůšek, Zdeněk Braunschläger, Officer of vigilance committee Milan Mach ![]() Marek - tram conductor Stanislav Fišerĭirector of transport undertakingğrantišek Hanus Music: Jiří Bažant, Jiří Malásek, Vlastimil Hála ![]()
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